Publications

2024

Loss of FIC-1-mediated AMPylation activates the UPRER and upregulates cytosolic HSP70 chaperones to suppress polyglutamine toxicity

Van Pelt KM, Truttmann MC

bioRxiv [preprint] 2024 Oct 9:2024.10.07.617097. doi: https://doi.org/10.1101/2024.11.27.625751.

PMID: 39651313

C. elegans behavior, fitness, and lifespan are modulated by AWB/ASH-dependent death perception

Hernandez-Lima MA, Seo B, Urban ND, Truttmann MC

bioRxiv [preprint] 2024 Oct 9:2024.10.07.617097. doi: https://doi.org/10.1101/2024.10.07.617097.

PMID: 39416137

Small molecule FICD inhibitors suppress endogenous and pathologic FICD-mediated protein AMPylation.

Chatterjee BK, Alam M, Chakravorty A, Lacy SM, Rech J, Brooks CL 3rd, Arvan PD, Truttmann MC.

bioRxiv [preprint]. 2024 Jul 16:2024.07.13.603377. doi: 10.1101/2024.07.13.603377.

PMID: 39071275 

FICD deficiency protects mice from hypertrophy-induced heart failure via BiP-mediated activation of the UPR ER and ER-phagy

Lacy SM, Taubitz RJ, Urban ND, Turowski SN, Smith ED, Helms AS, Michele DE, Truttmann MC

bioRxiv [preprint] 2024 May 30:2024.05.28.596287. doi: 10.1101/2024.05.28.596287.

PMID: 38853840

GRAIL1 stabilizes misfolded mutant p53 through a ubiquitin ligase-independent, chaperone regulatory function.

Ray P, Jaiswal S, Ferrer-Torres D, Wang Z, Nancarrow D, Curtin M, San Martinho M, Lacy S, Kasturirangan S, Thomas D, Spence J, Truttmann MC, Lagisetty KH, Lawrence T, Wang T, Beer D Ray D

Mol Cancer Res. 2024 Nov 1;22(11):996-1010. doi: 10.1158/1541-7786.MCR-24-0361

PMID: 39018356

TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections

Porter CM, Tabler S, Choi S,  Truttmann MC.

Scientific Reports 2024 Jun 3;14(1):12688. doi: 10.1038/s41598-024-63152-2

PMID: 38830987

Editorial: A new Chapter for Cell Stress and Chaperones

Bourboulia D, Blair LJ, Clark MS, Edkins AL, Hightower LE, Mollapour M, Prahlad V, Repasky EA, Truebano M, Truman AW, Truttmann MC, van Oosten-Hawle P, Woodford MR. 

Cell Stress Chaperones 2024 Feb;29(1):113-115. doi: 10.1016/j.cstres.2024.01.007.

PMID: 38309687

2023

A Meta-Analysis of RNA-Seq Studies to Identify Novel Genes that Regulate Aging

Bairakdar MD, Tewari A, Truttmann MC.

Exp Gerontol. 2023 Mar:173:112107. doi: 10.1016/j.exger.2023.112107

PMID: 36731807

2022

The AMPylase FIC-1 modulates TGF-β signaling in Caenorhabditis elegans

Henandez Lima MA, Champion, M, Matiola Z, Truttmann MC.

Front. Mol. Neurosci. 2022. https://doi.org/10.3389/fnmol.2022.912734

PMID: 36504677

2021

Explaining inter-lab variance in C. elegans N2 lifespan: Making a case for standardized reporting to enhance reproducibility

Urban ND, Cavataio JP, Berry Y, Vang B, Maddali A, Sukpraphrute RJ, Schnell S, Truttmann MC.

Exp Gerontol. 2021 Dec;156:111622. doi: 10.1016/j.exger.2021.111622.

PMID: 34793939

Deletion of mFICD AMPylase alters cytokine secretion and affects visual short-term learning in vivo.

McCaul N, Porter CM, Becker A, Tang CA, Wijne C, Chatterjee B, Bousbaine D, Bilate A, Hu CA, Ploegh H, Truttmann MC.

J Biol Chem. 2021 Aug 19:100991. doi: 10.1016/j.jbc.2021.100991. 

PMID: 34419450

Over-expression of the constitutive AMPylase FIC-1(E274G) does not deplete cellular ATP pools in C. elegans

Champion M, Truttmann MC.

MicroPubl Biol. 2021 Jun 25;2021:10.17912/micropub.biology.000409. doi: 10.17912/micropub.biology.000409.

Fic and non-Fic AMPylases: protein AMPylation in metazoans

Chatterjee BK, Truttmann MC.

Open Biol. 2021 May;11(5):210009. doi: 10.1098/rsob.210009

PMID: 33947243

2020

Post-translational modifications of Hsp70 family proteins: Expanding the chaperone code

Nitika, Porter CM, Truman AW, Truttmann MC.

J Biol Chem 2020 Jul 31; 295(31):10689-10708. doi: 10.1074/jbc.REV120.011666

PMID: 32518165

Caenorhabditis elegans as a model system for studying aging-associated neurodegenerative diseases

Van Pelt KM, Truttmann MC.

Translational Medicine of Aging; Volume 4, 2020, Pages 60-72

https://doi.org/10.1016/j.tma.2020.05.001

2018

Protection of tissue physicochemical properties using polyfunctional crosslinkers. 

Park YG, Sohn CH, Chen R, McCue M, Yun DH, Drummond GT, Ku T, Evans NB, Oak HC, Trieu W, Choi H, Jin X, Lilascharoen V, Wang J, Truttmann MC, Qi HW, Ploegh HL, Golub TR, Chen SC, Frosch MP, Kulik HJ, Lim BK, Chung K.

Nat Biotechnol. 2018 Dec 17. doi: 10.1038/nbt.4281. [Epub ahead of print]

PMID: 30556815

Chaperone AMPylation modulates aggregation and toxicity of neurodegenerative disease-associated polypeptides.

Truttmann MC, Pincus D, Ploegh HL.

Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5008-E5017. doi: 10.1073/pnas.1801989115. Epub 2018 May 14.

PMID: 29760078

2017

In vitro AMPylation Assays Using Purified, Recombinant Proteins.

Truttmann MC, Ploegh HL.

Bio Protoc. 2017 Jul 20;7(14). pii: e2416. doi: 10.21769/BioProtoc.2416.

PMID: 29170749

Site-Specific Protein Labeling via Sortase-Mediated Transpeptidation.

Antos JM, Ingram J, Fang T, Pishesha N, Truttmann MC, Ploegh HL.

Curr Protoc Protein Sci. 2017 Aug 1;89:15.3.1-15.3.19. doi: 10.1002/cpps.38.

PMID: 28762490

rAMPing Up Stress Signaling: Protein AMPylation in Metazoans.

Truttmann MC, Ploegh HL.

Trends Cell Biol. 2017 Aug;27(8):608-620. doi: 10.1016/j.tcb.2017.03.004. Epub 2017 Apr 19. Review.

PMID: 28433487

Hepta-Mutant Staphylococcus aureus Sortase A (SrtA7m) as a Tool for in Vivo Protein Labeling in Caenorhabditis elegans.

Wu Q, Ploegh HL, Truttmann MC.

ACS Chem Biol. 2017 Mar 17;12(3):664-673. doi: 10.1021/acschembio.6b00998. Epub 2017 Jan 18.

PMID: 28098972

Unrestrained AMPylation targets cytosolic chaperones and activates the heat shock response.

Truttmann MC, Zheng X, Hanke L, Damon JR, Grootveld M, Krakowiak J, Pincus D, Ploegh HL.

Proc Natl Acad Sci U S A. 2017 Jan 10;114(2):E152-E160. doi: 10.1073/pnas.1619234114. Epub 2016 Dec 28.

PMID: 28031489

2016

Recent advances in sortase-catalyzed ligation methodology.

Antos JM, Truttmann MC, Ploegh HL.

Curr Opin Struct Biol. 2016 Jun;38:111-8. doi: 10.1016/j.sbi.2016.05.021. Epub 2016 Jun 16. Review.

PMID: 27318815

The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.

Truttmann MC, Cruz VE, Guo X, Engert C, Schwartz TU, Ploegh HL.

PLoS Genet. 2016 May 3;12(5):e1006023. doi: 10.1371/journal.pgen.1006023. eCollection 2016 May.

PMID: 27138431

Corrigendum: Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.

Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL.

Nat Biotechnol. 2016 Feb;34(2):210. doi: 10.1038/nbt0216-210c. No abstract available.

PMID: 26849523

2015

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.

Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL.

Nat Biotechnol. 2015 May;33(5):538-42. doi: 10.1038/nbt.3190. Epub 2015 Mar 23. Erratum in: Nat Biotechnol. 2016 Feb;34(2):210.

PMID: 25798939

HypE-specific nanobodies as tools to modulate HypE-mediated target AMPylation.

Truttmann MC, Wu Q, Stiegeler S, Duarte JN, Ingram J, Ploegh HL.

J Biol Chem. 2015 Apr 3;290(14):9087-100. doi: 10.1074/jbc.M114.634287. Epub 2015 Feb 12.

PMID: 25678711

2014

Simultaneous analysis of large-scale RNAi screens for pathogen entry.

Rämö P, Drewek A, Arrieumerlou C, Beerenwinkel N, Ben-Tekaya H, Cardel B, Casanova A, Conde-Alvarez R, Cossart P, Csúcs G, Eicher S, Emmenlauer M, Greber U, Hardt WD, Helenius A, Kasper C, Kaufmann A, Kreibich S, Kühbacher A, Kunszt P, Low SH, Mercer J, Mudrak D, Muntwiler S, Pelkmans L, Pizarro-Cerdá J, Podvinec M, Pujadas E, Rinn B, Rouilly V, Schmich F, Siebourg-Polster J, Snijder B, Stebler M, Studer G, Szczurek E, Truttmann M, von Mering C, Vonderheit A, Yakimovich A, Bühlmann P, Dehio C.

BMC Genomics. 2014 Dec 22;15:1162. doi: 10.1186/1471-2164-15-1162.

2013

Bartonella henselae trimeric autotransporter adhesin BadA expression interferes with effector translocation by the VirB/D4 type IV secretion system.

Lu YY, Franz B, Truttmann MC, Riess T, Gay-Fraret J, Faustmann M, Kempf VA, Dehio C.

Cell Microbiol. 2013 May;15(5):759-78. doi: 10.1111/cmi.12070. Epub 2012 Dec 6.

PMID: 23163798

2011

Bartonella henselae engages inside-out and outside-in signaling by integrin β1 and talin1 during invasome-mediated bacterial uptake.

Truttmann MC, Misselwitz B, Huser S, Hardt WD, Critchley DR, Dehio C.

J Cell Sci. 2011 Nov 1;124(Pt 21):3591-602. doi: 10.1242/jcs.084459. Epub 2011 Nov 1.

PMID: 22045736

BID-F1 and BID-F2 domains of Bartonella henselae effector protein BepF trigger together with BepC the formation of invasome structures.

Truttmann MC, Guye P, Dehio C.

PLoS One. 2011;6(10):e25106. doi: 10.1371/journal.pone.0025106. Epub 2011 Oct 17.

PMID: 22043280

Combined action of the type IV secretion effector proteins BepC and BepF promotes invasome formation of Bartonella henselae on endothelial and epithelial cells.

Truttmann MC, Rhomberg TA, Dehio C.

Cell Microbiol. 2011 Feb;13(2):284-99. doi: 10.1111/j.1462-5822.2010.01535.x. Epub 2010 Oct 22.

PMID: 20964799

2009

A translocated protein of Bartonella henselae interferes with endocytic uptake of individual bacteria and triggers uptake of large bacterial aggregates via the invasome.

Rhomberg TA, Truttmann MC, Guye P, Ellner Y, Dehio C.

Cell Microbiol. 2009 Jun;11(6):927-45. doi: 10.1111/j.1462-5822.2009.01302.x. Epub 2009 Feb 27.

PMID: 19302579